Denise and everybody else interested in Flow Jo: I have been using Flow Jo for a year and my requirements are very much like yours. I also do a lot of DNA analysis, apoptosis and proliferation assays. We just use the Fl-1, Fl-2, etc. as defaults. That way we can run different set of samples using several different markers with the same dye at a single run. Later in the analysis we add to the plots the specific name of the markers. It is fast and efficient, I definitely love it, my analysis time has been cut down in an exponential way. You create a template and can drop buckets of samples in it to be analyzed in no time. This includes time for you to view every single plot in a movie sequence to be sure that all your gates are correct. Still if you have cells from different origins and need to correct gates, this can be done for the individual samples that require correction leaving the rest untouchable. And the results on excel are at your finger tips customized by your needs in a couple of clicks. I will never forget all the macros I needed to built to create individual excel tables from cell quest. I fewer words, as I told BD who where the ones who introduce me to Flow Jo: "Flow Jo is like to live in the future and there is no way you want to return to the past". Carmen "Manfra, Denise" wrote: > Hi: > > I wanted some feedback on the FLOW Jo application. I > currently do all of my analysis with CellQuest. I am wondering if FLOW Jo > may expedite my analysis. However, I do not use the same markers all the > time, and in one experiment I may be running more than one stain with > multiple different antibodies. Basically, very seldom do I run the same set > of markers. How does FLOW Jo work for such conditions?? How about plot > analysis and excel: apparently Excell FLOW Jo puts the data directly into > EXcell?? What has been your experience with FLOW Jo?? Any information would > be useful. > > denise > > *************************************************************** > This message and any attachments is solely for the intended recipient. If > you are not the intended recipient, disclosure, copying, use, or > distribution of the information included in this message is prohibited -- > please immediately and permanently delete this message.
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