Hi Maria,
You do not say what kind of secondary rabbit IgG you are using. This is
often the problem if you use a whole as apposed to F(ab') antibody. If that
is not the case, let me know.
Paula
At 03:49 PM 6/6/2001 -0300, admin wrote:
>I hope you can help me.
>I began working in immunofluorescence on sperm smears. The first
>antibody is a policlonal anti- bovine sperm membrane extract, developed
>in rabbits and the second antibody an anti rabbit IgG-FICT.
>The problem is that the sperm cells seem to have their own fluorescence
>which I could not remove treating the slides with ammonium clorhide, and
>both preimmune sera. Sperm is previously fixed with parafolmaldehide or
>glutaraldehide.
>maybe you can tell me how I can lower this fluorescence or who else I
>can ask. My e-mail adress is mmongiar@cicv.inta.gov.ar
>Thank you in advance.
>Sincerely yours,
>María Elena Mongiardino
>I. Patobiología
>CICV. INTA
Paula Fukushima
Flow Cytometry
LP, NCI, NIH
10 Center Drive MSC-1500
Bethesda, Md. 20892-1500
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