I hope you can help me. I began working in immunofluorescence on sperm smears. The first antibody is a policlonal anti- bovine sperm membrane extract, developed in rabbits and the second antibody an anti rabbit IgG-FICT. The problem is that the sperm cells seem to have their own fluorescence which I could not remove treating the slides with ammonium clorhide, and both preimmune sera. Sperm is previously fixed with parafolmaldehide or glutaraldehide. maybe you can tell me how I can lower this fluorescence or who else I can ask. My e-mail adress is mmongiar@cicv.inta.gov.ar Thank you in advance. Sincerely yours, María Elena Mongiardino I. Patobiología CICV. INTA
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:22 EST