I would like to comment that in some cases, we have seen some vibration related issues but one must ask the question, "what am I going to look at with my cytometer"? If you are looking at sperm like we are which can be as low as 2.8% difference between X and Y sperm peaks and we run in linear scale, vibration can be an issue. Although I would not build a new laboratory because of it. We solved it by the use of rubber feet. If you are going to run log signals, I would have to assume that some vibration will not affect you at all. Mike -----Original Message----- From: J.Paul Robinson [mailto:jpr@flowcyt.cyto.purdue.edu] Sent: Thursday, May 24, 2001 8:08 PM To: cyto-inbox Subject: Re: How much vibration is a problem? Well at the risk of being unscientific and ignorant....I am going to weigh in on this one... 23 years ago when we bought our first cytometer..we were told "you must have it in the basement....vibration will kill you....etc etc" ...we put it on the 2nd floor of a 100 year old building, surrounded by busy roads.....no problems At Michigan when I was there they renovated the entire basement and created a beautiful flow unit, but a dungeon.... Here at Purdue - before I came here, someone decided that the flow had to be in the basement.....we have beautiful lab, no windows, bla, bla...and most of us are starting to look like moles...(well I am anyway) I think 99% of the perception that vibration is a problem is overdone......pick the corner lab, with the best view and go for it... Paul Robinson (Committee for prevention of cruelty to flow lab people) Date sent: Wed, 23 May 2001 17:12:27 +0100 From: Richard Grenfell <rlg@mrc-lmb.cam.ac.uk> To: cyto-inbox Subject: How much vibration is a problem? Hi everyone I'm new to the list and new to cytometry. We have a Cytomation MoFlo and are considering moving it to another lab (larger hopefully!) The question of vibration has come up. Now, not being used to these instruments, I wondered if anyone here could help. How would I go about measuring vibration in a lab and how much is 'too much'. Also, as a general rule of thumb, is it best siting these instruments as low down in a building as possible, though fairly near a road, or higher up in the building? Any help would be appreciated. Thanks Richard Grenfell -- Richard Grenfell Laboratory of Molecular Biology Medical Research Council Cambridge rlg@mrc-lmb.cam.ac.uk J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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