Hi all, I'd like to weigh in with the Guava perspective of what we're introducing, so as to avoid any confusion. The Guava PC (Personal Cytometer) is a new system for cellular analysis that is actually radically different than conventional cytometers, both conceptually and in practice. For example, the Guava PC does not use sheath fluid, enabling the system to be very compact in design and to enable the use of small sample volumes (50ul minimum volume) and/or low concentrations of cells (down to 10,000 cells/ml, final concentration). It uses a solid-state laser with an emission wavelength of 532nm (it's a frequency doubled YAG) and is used with fluorochromes that are compatible with that wavelength. The system measures relative particle size (forward angle light scatter) and two fluorescences. It does indeed come with a laptop computer and data can be stored in the most recent version of the FCS datafile format (FCS 3.0). We develop applications as what we call "integrated solutions." This means we've identified the optimal instrument settings for each application and allow those to be easily re-established, optimally formulated the reagents and developed software that automate data analysis, with results immediately exported to a spreadsheet. The applications that are available now include automated cell counting and viability determinations (no more manual cell counts or Trypan blue exclusion counts for viability), protein expression analysis (e.g., antibody screening) and apoptosis analysis. Others will be introduced in the future. The system is available now (we've shipped several) and can be demonstrated for the applications that I've mentioned. Please feel free to contact me with any questions you have. Best Regards, Jeff Harvey Guava Technologies, Inc. -----Original Message----- From: J.Paul Robinson [mailto:jpr@flowcyt.cyto.purdue.edu] Sent: Friday, May 18, 2001 1:14 PM To: cyto-inbox Subject: Re: GuavaPC I saw the instrument at Cell Biology I think in December - I did not see it actually running at that time. It seemed to have some nice features - such as running entirely on a laptop which was neat. It seemed to be a capable instrument for the tasks they were identifying - This is a relatively new company trying to break into a tough field so it will be interesting to see how they go. I have not actually used one so I can't comment more than that. Paul Robinson From: "Topham, David" <David_Topham@URMC.Rochester.edu> To: cyto-inbox Subject: GuavaPC Date sent: Fri, 18 May 2001 09:26:35 -0400 We just received a flyer describing an inexpensive cytometer. It seems to have limited capabilities, but still does at least two-colors, and sits on the benchtop. It is being sold by a company based in Burlingame, CA. Out of curiosity, does anyone out there know about this instrument, and what can you tell us about it? Thanks. David J. Topham, Ph.D. David H. Smith Center for Vaccine Biology & Immunology Aab Institute for Biomedical Sciences University of Rochester Medical Center 601 Elmwood Avenue, Box 609 Rochester, NY 14642-8609 Tel. 716 273-1403 FAX 716 273-2452 E-mail: david_topham@urmc.rochester.edu J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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