In our experience, which is at least 5 years, there is no real problem regulating off of UV, but my guess is that this is mainly because most 488 parameters are measured in log. We see absolutely no observable variations in the 488 fluorescences while regulating off of the UV line. In terms of power, yes, if you regulate UV you have no control over the 488 output except to use ND filters, but this is really a pretty simple fix. In my opinion, the current Enterprise is a great laser (the early ones had a lifespan that could best be described as unacceptable...) if you want to minimize expenditures (and laser bench space) while maximizing your flexibility. Howard >I would like to request info from people who use/know the Enterprise laser >from Coherent. I have always been uneasy with using this laser for >simultaneous 488 and UV primarily because the laser is light regulated on >only one of the lines at a time. Thus, I worried that over time the >unregulated line might change and affect experimental results. Also for >the unregulated line you just get whatever you want based on what power >output you want from the regulated line (is this correct?). Short of using >filters to reduce the power if needed I assume this is the >situation. However, I have no direct experience and am now considering a >system with that laser. What has the experience been? Is this a real >worry but not actually realized? What is the lifetime ratio of the 488/UV >lines? I appreciate your advice and thanks in advance. > >Larry > > >Larry W. Arnold, Ph.D. >Res. Assoc. Prof. >Director, Flow Cytometry Facility >Department of Microbiology and Immunology >Lineberger Comprehensive Cancer Center >CB# 7290 >University of North Carolina at Chapel Hill >Chapel Hill, NC 27599 >Phone: 919-966-1530 >FAX: 919-962-8103 -- Howard T. Petrie, Ph.D. Head, Laboratory of Developmental Immunology Memorial Sloan-Kettering Cancer Center Box 341, 1275 York Avenue New York, NY 10021 phone (212)639-2149 fax (212)794-4019
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