I would like to request info from people who use/know the Enterprise laser from Coherent. I have always been uneasy with using this laser for simultaneous 488 and UV primarily because the laser is light regulated on only one of the lines at a time. Thus, I worried that over time the unregulated line might change and affect experimental results. Also for the unregulated line you just get whatever you want based on what power output you want from the regulated line (is this correct?). Short of using filters to reduce the power if needed I assume this is the situation. However, I have no direct experience and am now considering a system with that laser. What has the experience been? Is this a real worry but not actually realized? What is the lifetime ratio of the 488/UV lines? I appreciate your advice and thanks in advance. Larry Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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