Wolfgang, The only advantages of Oregon Green 488 and Alexa 488 are that they are more photostable than FITC. If photostability is not an issue and you are able to get good results with FITC, there is little reason to switch to these much more expensive, proprietary dyes. One altrnative to FITC is 5,(6)-carboxyfluorescein, NHS ester. This is an amine-reactive fluorescein that results in a more stable (amide) linkage than the thiourea bond formed when labeling amines with fluorescein isothiocyanate. Best regards, Michael Kuhn, President Helix Research Fluorescence Chemistry mkuhn@helixresearch.com www.helixresearch.com Phone: 541 988 0464 FAX: 541 744 9179
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