sorting BAEC cells

From: Elaine Kunze (mek4@psu.edu)
Date: Tue May 15 2001 - 08:39:33 EST


I am trying to sort bovine aortic endothelial cells using labelled LDL.
Recently we are getting fibroblast contamination.  The sort seems to go
well, >99.5% fall in the chosen gates for the highest LDL and a ss-fs gate.
 Is there something else I can do?  What about a second label which is
positive for fibroblasts.
****************************************************************************
	Elaine Kunze
	Flow Cytometry.....Image Analysis...
	Life Sciences Consortium
	8B Althouse Laboratory	(814-863-2762)
	Penn State University
	University Park, PA 16802



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