Danni,
Your problem is most likely a pressure problem with the sheath tank air
pressure or the sample tube air pressure or both.
Turn on your cytometer and boot your computer. Launch Cellquest, connect
to the cytometer and open the status page. Note your laser power and
current, as well as sample voltage. In the first five minutes, your laser
power should be 15mW and your laser current should be approx. 7 amps. Prime
the instrument and observe the fluid flow through your flow cell and the
waste (orange) line of your waste tank. After the five minute warm up
period, and when your sheath tank has pressurized, put the sample tube
holder to the side and take a reading of the the sample voltage on your
status page. It should be approx. 10.23 volts. Also put a sample tube with
DI water on the instrument and run on high. Check the sample voltage. It
should be approx. 5.9 volts. Change the flow rate to low and sample voltage
should change to approx. 6.3 volts.
With any pressure problem first ensure that your sheath tank has no
leaks, and change your bal-seal. After you have tried these things and made
note of your findings, please contact me so that we may isolate your problem
to a specific area.
Regards,
Raymond Lannigan
Tritech Inc.
2014 Renard Ct. Suite I
Annapolis, MD 21401
1-410-266-1522
1-800-886-7004
www.tritechinc.com
-----Original Message-----
From: Dhanwanthie Ramduth <Ramduthd1@nu.ac.za>
To: cyto-inbox
Date: Friday, May 11, 2001 5:31 PM
Subject: facs calibur
>
>dear flow cytometrists
>
>we have been experiencing a problem with our facs calibur recently. the
cytometer cannot
>switch from the standby to ready mode. when attempting to acquire data, it
always
>comes up withn a warning message " cytometer status not ready". we have
folowed the
>trouble shooting guide, and and always ensure taht the machine is
pressurised, and all
>connections are intact. what could be the cause of this problem? and how do
we solve it?
>
>kind regards
>danni
>
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