Cheryl,
Perhaps someone at Beckman-Coulter can verify . . .
I believe that since their XL uses what they call "Digital Signal Processing,"
essentially look-up tables for the signals from the detectors, linearity is not an issue
- - unless the calibration is off, and that's something only their service engineers
can deal with ('course, iyou can do a lot of damage with a set of bootleg schematics
and a mini-screwdriver . . .).
If you're worried about the detectors, I think the ones used today are designed to be
linear across a broad range of voltage. But, again, that's something you can check
with Coulter.
Why are you worried, anyway?
MAK.
--
Mark A. KuKuruga, Managing Director
University of Michigan Flow Core
7416 CCGC 0946
(734) 647-3216, fax (734) 936-7376
kukuru@umich.edu
>>> "Torretto, Cheryl" <Cheryl.Torretto@pfizer.com> 05/11/01 07:53AM >>>
Hi all,
I did a quick search on the Purdue archived e-mail and couldn't find any
previous discussions on this topic, so here's my question.
What is the best way to check the linearity of the XL? Since there are no
log amplifiers, is it worth it to use Immunobrite as an indirect measure? I
have also used a method by Bagwell (Cytometry 1989) to check the linearity
of the linear amps. I'm curious to know what other people are doing.
Thanks,
Cheryl Torretto
Pfizer Global Research and Development
Mississauga, ON
CANADA
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