I suggest that you use antibodies to detect CD71 for isolating activated T cells.
Since it is the transferrin receptor responsible for bringing iron into activated and
proliferating cells, it becomes upregulated and expressed on the surface of leukocytes
(T cells, B cells, and macrophages) as they leave G0 into G1.
Whereas CD69 becomes expressed on the same cell types during activation, it
becomes progressively downregulated through successive divisions. In contrast, CD71
expression remains elevated during proliferation. Although CD25 measures expression of
a unit of the IL-2 receptor that generally correlates with activation of
IL-2-responsive cells, its expression is variable depending on the subcategory and
differentiation status of the cell type.
Kevin G. Waddick, Ph.D.
Parker Hughes Cancer Center
2657 Patton Road
St. Paul, MN 55113
Heather Medbury wrote:
> Hi FLOWers,
>
> We are doing mixed lymphocyte reactions and want to detect the activated T cells by
> flow cytometry, so we can examine them for cytokine production.
>
> asking around people have suggested we stain for either CD25, CD69 or CD71.
>
> I am not familiar with these markers, do they appear early or late, after how many
> hours etc.
> and any suggestions on the best time points to look for cytokines.
>
> Heather
>
> Heather Medbury (PhD)
> Department of Surgery
> Westmead Hospital
> Westmead 2145
> 61-2 9845 7677
> --------------------------------------------------------------
> All experiments are preliminary
>
> ------------------------o0)(0o--------------------------------------
>
> "In his heart a man plans his course
> but the Lord determines his steps"
>
> ---------------------------------------------------------------
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