Hi FLOWers,
We are doing mixed lymphocyte reactions and want to detect the activated T cells by
flow cytometry, so we can examine them for cytokine production.
asking around people have suggested we stain for either CD25, CD69 or CD71.
I am not familiar with these markers, do they appear early or late, after how many
hours etc.
and any suggestions on the best time points to look for cytokines.
Heather
Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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All experiments are preliminary
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"In his heart a man plans his course
but the Lord determines his steps"
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