Jackie: Do you have access to a sorter? The best way we've found to circumvent this problem is to use a sorter and place an ND filter in front of the Fluorescence detector. Joan From: Jacqueline.Saleh@aventis.com To: cyto-inbox Subject: GFP/YFP Date: Wed, 2 May 2001 14:32:18 -0500 X-PMFLAGS: 34078848 0 1 30574.cnm Hi Flowers, When running GFP/YFP the GFP is so bright it tails off into the double positive region. How can I get rid of this problem without over compensating? Thanks, Jackie Saleh Aventis Pharmaceuticals jacqueline.saleh@aventis.com -- ************************** :0) ********************************** Joan M. Batchelder Instrumentation Specialist Flow Cytometry Lab UW Clinical Cancer Center, K4/535 600 Highland Ave. Madison, WI 53792 (608) 263-0313
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