>Hi Flowers, > >When running GFP/YFP the GFP is so bright it tails off into the double >positive region. How can I get rid of this problem >without over compensating? > >Thanks, Jackie Saleh >Aventis Pharmaceuticals >jacqueline.saleh@aventis.com Hi, Jackie, Did you try turning down the laser to 50 mW and putting both an ND and narrow band pass filters in front of your detectors if you are using a sorter? Kathy -- -------------------------------------------------------------- Kathy Schell Supervisor, UWCCC Flow Cytometry Facility 600 Highland Ave. K4/535 Madison, WI 53792 Voice: 608-263-0313 e-mail: kschell@facstaff.wisc.edu
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