We too use beads to align the instrument on a daily basis. This "habit" stems from our early days in flow when Ortho provided a particle suspension to align the forward scatter parameter. We extended this to also include fluorescence parameters. There are many good bead products on the market for this purpose. These are especially useful in the research setting where optimization of several parameters needs to be performed several times a day. Especially when using multiple lasers or changing excitation wave lengths . Charles A. Kuszynski, Ph.D. Assistant Professor/Director Cell Analysis Facility University of Nebraska Medical Center 986495 Nebraska Medical Center Omaha, NE 68198-6495 402 559-6267 voice 402 559-4077 fax ckuszyns@unmc.edu
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