>I have done some work in this area and it is very straightforward flow. >The cells from blood are very similar in size to other animals-especially >feline and pig blood. You may have to make some adjustments in the >forward scatter and they may be more granuular then you are use to. Make >sure to to fine out from the researcher exactly what you are looking >for-my people look for macrophages and they are very granular in fish. I >also believe my researchers use 2% freshly made paraformaldehyde to fix >the cells and then stain for PI. I will dig through my data banks and >look for examples to send to you. I work on BD machines, FACSCalibur and >FACScan. Hope this helps. > >good luck. > >Janet Dow > > > > >>Fish cells. >> >>This is a new area for me. Does anybody have any data files to look at or >>protocols to use for the fixing of fish cells to stain with PI when looking >>at the DNA content of the cells. It would be nice to know what I should be >>looking at and the gating strayegies to achieve the cell cycle profile. >> >>Any help or direction would be appreciated. >> >>Andy > > > Janet Dow Research Technician and Manager Flow Cytometry Facility North Carolina State College of Veterinary Medicine Room C-314 Raleigh, NC 27606 (919)513-6364
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