Re: PE-Cy5 in the NOD

From: Julie Auger (jauger@flowcity.bsd.uchicago.edu)
Date: Tue Apr 24 2001 - 16:43:44 EST


Mark -
It seems this link only advertises anti-human antibodies.  It has been seen
by many labs, including mine, that PE-Cy5 conjugates bind to  B cells from
the NOD mouse strain, which Keith is referring to.

Julie
P.S.  I also do not believe this is an Fc receptor binding, as we routinely
use an antibody against Fcgamma II/III Receptor (clone 2.4G2) when we stain.

At 09:23 AM 4/24/01, you wrote:

>Keith,
>So, are you disputing Caltag's claim? (see < www.caltag.com > ; "New Tri-Color
>Conjugates" link).
>MAK.
>
>
>--
>Mark A. KuKuruga, Managing Director
>University of Michigan Flow Core
>7416 CCGC 0946
>(734) 647-3216, fax (734) 936-7376
>kukuru@umich.edu
>
>
> >>> Keith Bahjat <kbahjat@ufl.edu> 04/21/01 02:59PM >>>
>
>Joe and others,
>
>As far as the binding of Cy5 to NOD B cells goes, I can tell you the new
>formulations of PE-Cy5 DO NOT correct this problem . . .



This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:16 EST