Hello, I am interested in quantifying chemokine receptors on lymphocytes. We have a BD FACScan attached to a HP platform (HP 310 with consort 30). I was advised that I could convert the files I save on the HP computer into MSDOS with HPCOPY and to do the quantification with WIN MDI 2.8. I have downloaded the software and for starters tried to convert files I have saved with the Simulset software (for CD4 counts). The files are saved with extensions .001,.002 etc. I started off with the first file and gated the lymphocytes, and when I open the second file, it failed to open (saying it is not in FCS1/FCS2 format). I am howwever able to read all the files with an extension in odd numbers. I tried to change the extensions of the files that end in even numbers to odd numbers but am still unable to read the files. I have only tried to read files that I have saved with Simulset. I have not yet done any quantitative analysis, and would be obliged if you could guide me. If there is any written matter anywhere, I would be happy to read it. With warm regards, Sandeep. ===== Dr. Sandeep Ramalingam, Department of Clinical Virology, Christian Medical College Hospital, Vellore-632004, India. Phone(off): 91-416-222102, ext 2070 Fax: 91-416-232035 Email: sandeeprk@yahoo.com ____________________________________________________________ Do You Yahoo!? Get your free @yahoo.co.uk address at http://mail.yahoo.co.uk or your free @yahoo.ie address at http://mail.yahoo.ie
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