Colleagues - A researcher here is interested in tracking nuclear translocation of a protein. Is there a good method for this by flow? He is looking to see if this translocation occurs in only a subset of cells. I suggested antibody staining, followed by fixation and nuclear isolation. With this approach there is the possibility of cytosolic contamination. We discussed the possibility of a nuclear stain that would quench the fluorescence of a tagged protein when it translocates. Any suggestions? Thanks, Julie
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