I have a user that has had this problem. It seems that spinning down the
isotype antibody at high speed before staining helps reduce background.
Presumably by pelletizing precipitates.
Paul
> From: "Douglas S. Smoot" <rin0dss@bumed30.med.navy.mil>
> Date: Thu, 12 Apr 2001 16:10:03 -0400
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: PE-Cy5 Backgrounds
>
>
> Just a quick questions. I have had trouble phrasing the search properly of
> the e-mail discussions, but there was a discussion a few years ago
> concerning high backgrounds for PE-Cy5 staining. (The samples are coming
> up positive with Isotype staining - I know, Isotype staining might not be a
> true negative, but that is a different discussion. :-)) Is there a solution
> for the high background other than using some other fluorochrome like PerCp
> which doesn't have this problem, as I understand it. Thank you in advance
> for your help.
>
> Doug
> {-----------------------------------------------------------------------}
> Douglas Smoot
> NIDDK-Navy Transplantation & Autoimmunity Branch
> Naval Medical Research Center
> AFRRI Building 46, Room 2415
> 8901 Wisconsin Avenue
> Bethesda, MD 20889-5603
> voice: 301-295-1843
> fax: 301-295-6484
>
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