Hello everybody, we want to analyse the expression of interleukins in monocytes. During the first analysis, a lot of questions appeared. Therefore, I asked some people and got no really clear answers. Hopefully, somebody can help me. 1) We want to stimulate whole blood for up to 24 hours and analyse the monocytes afterwards. In which sort of tubes should we keep the blood or is it possible to block the attachment of the monocytes to the tube material? 2) If the monocytes are attached to the wall, is it possible to include them in an analysis or are all of them stimulated by the attachment per se? 3) We see that one have to predilute the blood in RPMI or AIM-V medium before one stimulate the cells. Which medium is the better one and why do one have to dilute it? 4) Which heparin blood is the better one, Li-heparin or Na-heparin? I see the question from yesterday and hope to get the answer as well. 5) Do anybody know a source of IL-18 antibodies for intracelluilar staining? Thanks for your help in advance Andreas Simm
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