Post sort viability was always better with the cells being 60-70% confluent. Jim Houston -----Original Message----- From: Marty Bigos [mailto:mbigos@gladstone.ucsf.edu] Sent: Sunday, April 01, 2001 1:47 PM To: cyto-inbox Subject: Cell Lines Pre and Post Sort Hi- A grad student here asked me the following question. I couldn't come up with any hard evidence from my experience so I would like to ask for any feedback that others may have. Thanks. Marty Bigos Gladstone Flow Core "I was wondering what you knew about the affect of sorting a cell line on the health of the cells. Is it thought that you get healthier cells out post-sort if the cells start from a confluent or not yet confluent plate? Does it matter?"
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