Hi What sheath fluid are you using, some commercial sheath fluid is rather toxic to cells, I use the Biosure preservative free stuff and am pleased with it. You can just make up some PBS and use that. Are you giving the cells some protein before and after sorting, BSA before and FCS after, FCS can make cells clumpy so is often avoided before sorting. Good luck Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> Gilbert Pitts <grpitt2@pop.uky.edu> - 3/23/2001 9:10 AM >>> Hi Everyone, I have recently tried to sort GFP-identified cells from the mouse suprachiasmatic nucleus. Unfortunately, the cells appear to be dead after the FACS sort is completed. I've been using a trypsin dispersion is HBSS (supplemented with Hepes and pen/strep). Does anyone have any ideas of how I can increase cell viability? Thanks in advance! Gilbert -- Gilbert Pitts, Ph.D. Post-doctoral Scholar Dept. of Physiology University of Kentucky 800 Rose St, Room MS508 Lexington, KY 40536-0084 Tel. (859) 257-1645 Fax: (859) 323-1070
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