Hi Derek, To further phenotype this B220+IgM- population in your knockout mice, you may want to use anti-mouse CD19 as a more specific marker for B lineage and check to see if it is CD3+. The B220+IgM- population that you see may not be B cells; in normal mouse, B220 is expressed on activated NK and T cells (LAK cells) as well as BM progenitor cells of the NK lineage (see ref. below.) If these are immature B cells, markers to look at include CD43, BP-1, and CD24. If it turns out that this population is CD19- (i.e. non-B lineage), relevant T and NK markers such as CD3, DX5, PK136, CD94 may be the appropriate markers to look for. The papers reporting B220 expression on LAK and NK progenitors are: Rolink A, ten Boekel E, Melchers F, Fearon DT, Krop I, Andersson J. A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors. J Exp Med. 1996 Jan 1;183(1):187-94. Ballas ZK, Rasmussen W. Lymphokine-activated killer cells. VII. IL-4 induces an NK1.1+CD8 alpha+beta- TCR-alpha beta B220+ lymphokine-activated killer subset. J Immunol. 1993 Jan 1;150(1):17-30. Ballas ZK, Rasmussen W. Lymphokine-activated killer (LAK) cells. VI. NK1.1+, CD3+ LAK effectors are derived from CD4-, CD8-, NK1.1- precursors. Cell Immunol. 1991 May;134(2):296-313. Best regards, Lily eBioscience 5893 Oberlin Dr. San Dieo, CA 92121 http://www.ebioscience.com ----- Original Message ----- From: Derek Schulze <flow@post.queensu.ca> To: cyto-inbox Sent: Wednesday, March 21, 2001 1:10 PM Subject: b220+IgM- in mouse spleen > > We are seeing an increase of a B220+ IgM- population in the spleen of our > knock out mice. Are there any appropriate markers for mice to determine if > these are mature or immature B cells? > > Would any of the following be appropriate? > CD2 (LFA-2) > IgD > CD80 (B7-1) > CD86 (B7-2) > Derek Schulze > > Cancer Research Labs > Queen's University > 353 Botterell Hall > Kingston, ON, K7L 3N6 > (613) 533-6635 > > http://meds.queensu.ca/medicine/crl/ > >
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