Derek: Most likely these cells are activated gd T cells. (Did you observe this in response to infection?) In my previous lab we determined B220 to be an activation marker on gd T cells. This occurred in any strain of mouse. Try TCR1 vs B220! :0) For more information, see: van der Heyde, Elloso, Chang, Pepper, Batchelder and Weidanz (1996) "Expansion of the gd T cell subset in vivo during bloodstage malaria in B cell-deficient mice." Journal of Leukocyte Biology, volume 60, p. 221-229. Joan We are seeing an increase of a B220+ IgM- population in the spleen of our knock out mice. Are there any appropriate markers for mice to determine if these are mature or immature B cells? Would any of the following be appropriate? CD2 (LFA-2) IgD CD80 (B7-1) CD86 (B7-2) Derek Schulze Cancer Research Labs Queen's University 353 Botterell Hall Kingston, ON, K7L 3N6 (613) 533-6635 http://meds.queensu.ca/medicine/crl/ -- ************************** :0) ********************************** Joan M. Batchelder Instrumentation Specialist Flow Cytometry Lab UW Clinical Cancer Center, K4/535 600 Highland Ave. Madison, WI 53792 (608) 263-0313
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