So both Caltag and DAko MAB's were positive, with and without washing. We have not seen any case with this kind reactivity. I guess that it would be interesting to check CD79a cytoplasmic expression - although I assume it would be negative, since the phenotype was otherwise clearly T cell. Best wishes Anna At 14:06 2001-03-21 -0600, you wrote: >In this case, the CD19 showed up convincingly in 4 different tubes >(CD10F/CD19PE/CD45PerCP; Caltag, CD45PerCP/kappaF/CD19PE; Dako, >CD45PerCP/lambdaF/CD19PE; Dako, and kappaF/lambdaPE/CD19Cy5; Dako). The 3 >tubes containing surface Ig's were washed prior to staining. The CD10/CD19 >tube was not washed prior to staining. To me, it seems pretty convincing >that CD19 is being aberrantly expressed by these abnormal T/NK cells. Thanks >for your input and any other suggestions you may have. > >-----Original Message----- >From: Anja Porwit [mailto:Anja.Porwit@ks.se] >Sent: Wednesday, March 21, 2001 1:57 AM >To: Cytometry Mailing List >Subject: Re: Interesting case > > > > >This is very unusual. Which MAb/conjugate do you use? Have you checked if >this expression can be seen with other anti CD19 MAb's? >Kind regards >Anna >At 13:22 2001-03-19 -0600, you wrote: >> >>We recently received a peripheral blood sample on an 80 year old gentleman >>with mild leukocytosis and an absolute lymphocytosis (WBC-13,700/ul, 70% >>lymphocytes). Many of the lymphocytes had the morphology of LGLs. The >>lymphocytes showed expression of CD8, CD3, CD7, CD11c and CD16 without >>significant expression of CD4, CD2 or CD5. The phenotype is good for the >>T-cell type of LGL. Interestingly, the cells also showed relatively bright >>expression of CD19. I found a couple of case reports describing similar >>cases with CD20 but not with CD19. Has anyone else seen this in their >>personal experience or documented in the literature? Thanks in advance for >>your input. >> >> >>Christopher S. Bee, M.D. >>Medical Director, Cellular Immunology and Flow Cytometry >>Wilford Hall Medical Center >>Lackland AFB, Texas >>(210) 292-5455 >>DSN 554-5455 >>christopher.bee@59mdw.whmc.af.mil >> >> >> >Anna Porwit >Hematopathology Lab. >Department of Pathology, Radiumhemmet >Karolinska Hospital, 171 76 Stockholm, Sweden >Anja.Porwit@ks.se >tel.:+46-851774518 >fax.:+46-851775843 > Anna Porwit Hematopathology Lab. Department of Pathology, Radiumhemmet Karolinska Hospital, 171 76 Stockholm, Sweden Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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