Dear group, I wonder if there is a set of rules by which cells are defined in flow. For example "a monocyte will be defined as having forward and side scatter values of such and such and will also be positive for CD33 or 14 as well as 45". I am asking because I noticed a lot of application notes say monocytes are defined by side scatter vs. CD14/33 gating and I just don't feel comfortable with that, esp. in activated cells. I did some playing around with different markers and there usually are a few distinct populations based on the parameters above. Not to mention Propidium Iodide. Any thoughts will be appreciated, maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Get email at your own domain with Yahoo! Mail. http://personal.mail.yahoo.com/
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