hello everybody, what do you use as a negative controll (cells not expressing the marker) in FACS-analyses? Cells stained with the isotype or unstained cells? I started stainig cells for FACS-analyses for about 2 years and I always used isotypstained cells as a negetive controll (=marker for unspecific binding and for correcting the compensation). Someone told me, that Isotypestaining is not a good negative controll because the Isotype is from a different host, and can`t representate the unspecific binding. So it would be better to use unstained cells. What does you use/Think about this ? Thanks Kerstin ***************************************** Kerstin Beck Institut für Medizinische Mikrobiologie und Hygiene Abteilung Virologie Hermann-Herder-Strasse 11 D-79104 Freiburg Telefon : +49-(0)761-203-6646 Fax : +49-(0)761-203-6603 beckk@ukl.uni-freiburg.de *****************************************
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