John- BD sells a positive control for IC cytokine staining. They're called HiCK cells, numbers 1-4, depending on which cytokine you're interested in controlling for. HiCK-1 is the one you want for TNF-a and HiCK-3 is for IL-12. cheers- Regina Regina H. Harley, M.S. Lab Supervisor - Flow Cytometry Facility Center for Vaccine Development University of Maryland - Baltimore 685 West Baltimore Street Baltimore, MD 21201 (410) 706-7376 FAX: (410) 706-6205 rharley@medicine.umaryland.edu >>> "JOHN MARIO GONZALEZ _ PROFESOR DPT. MICROBIOLOGIA-." <jmgonzal@javeriana.edu.co> 03/17/01 09:41AM >>> One of my colleges is working with IC cytokine staining for IL-12 and TNFa in PBMC monocyte-depleted . They are having too much problems to identify cytokine secreting cells. a) They need a positive control for IL-12 and TNFa in lymphocytes. b) There is a mitogen or antigen that stimulate these cytokine secretion in lymphocytes. c) What are the relative percentage of IL-12 and TNFa secreting lymphocytes in peripheral blood. Thanks again. JOHN MARIO GONZALEZ M.D., Ph.D. Facultad de Ciencias Pontificia Universidad Javeriana Phone 00 571 2850503 Fax 00 571 3208320 Ext 4085
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