From: Bob Leif
To: cyto-inbox
You wrote
1) Are there sheath flow cell designs suitable for use with a high
magnification, high N.A. objective? I.e. with a thin wall at the
detection lens side. (This must be closed cell cytometers, not
cell sorters.)
I presume that you want a thin wall in order to use a high N.A. objective
with a short working distance. Unless you couple your objective with a fluid
to the transducer, you will be limited by the critical angle of the
air-glass (fused silica) interface. A spherical surface permits the use of
long working distance objective and a reasonably high N.A. for a square
interior flow cell (1,2). The N.A. can be increased for a triangular flow
cell; however, a triangular cross-section presents significant problems with
light-scatter detection.
I presently am not manufacturing transducers. However, my monolithic spheres
are off-patent (3). Other designs where a hemisphere is glued on also can
work. Monolithic designs can be built with small diameters, which permit the
use of higher N.A. long working distance objectives.
1. R. C. Leif and M. Wells; “Optical Analysis of the AMAC IIIS Transducer”.
Applied Optics, 26 pp. 3244-3248 (1987).
2. R. C. Leif, M. L. Cayer, W. Dailey, T. Stribling, and K. Gordon, “The use
of a Spherical Multiparameter Transducer for Flow Cytometry”. Cytometry 20,
pp 185-190 (1995).
3. R. C. Leif, "Orifice Inside Optical Elements", U.S. Patent 4,348,107
(1982).
-----Original Message-----
From: Emmanuel Gustin [mailto:Emmanuel.Gustin@Tibotec.be]
Sent: Wednesday, March 14, 2001 6:11 AM
To: cyto-inbox
Subject: Flow cell
Dear All,
We are trying to develop an application which might use the flow
cell and system of a flow cytometer. What I would like to know is:
1) Are there sheath flow cell designs suitable for use with a high
magnification, high N.A. objective? I.e. with a thin wall at the
detection lens side. (This must be closed cell cytometers, not
cell sorters.)
2) Can I buy these anywhere, as spare or second-hand parts?
How much would they cost?
3) Or does someone have a second-hand flow cytometer on offer
which contains suitable elements?
Best regards,
Emmanuel Gustin
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