Just a historical note. I believe the old Ortho CytoFluorografs used a
design like that. They used long working distance 20 or 40X objectives in
their later designs. They used these objectives for their light gathering
power to increase sensitivity, or at least, so I was told. They used their
standard flowcells for these applications.
2) Ortho doesn't sell these anymore. (I miss my old Cytofluorograf.)
I don't know if this answers your question, but I hope it helps.
Doug
At 03:10 PM 3/14/01 +0100, Emmanuel Gustin wrote:
>Dear All,
>
>We are trying to develop an application which might use the flow
>cell and system of a flow cytometer. What I would like to know is:
>
>1) Are there sheath flow cell designs suitable for use with a high
> magnification, high N.A. objective? I.e. with a thin wall at the
> detection lens side. (This must be closed cell cytometers, not
> cell sorters.)
>
>2) Can I buy these anywhere, as spare or second-hand parts?
> How much would they cost?
>
>3) Or does someone have a second-hand flow cytometer on offer
> which contains suitable elements?
>
>Best regards,
>
>Emmanuel Gustin
>
{-----------------------------------------------------------------------}
Douglas Smoot
NIDDK-Navy Transplantation & Autoimmunity Branch
Naval Medical Research Center
AFRRI Building 46, Room 2415
8901 Wisconsin Avenue
Bethesda, MD 20889-5603
voice: 301-295-1843
fax: 301-295-6484
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