Hi Jacqueline, I can't say what is the best combination for you but perhaps you could try doing cotransfections with EBFP and EGFP/YFP and excite EBFP with an UV laser and EGFP/YFP with 488nm. Best wishes, Mika Jacqueline.Saleh@aventis.com said: > Dear Flowers: > I have an investigator here who is doing cotransfection using YFP/RFP. Under > the microscope he can clearly see the positive RFP cells. However maximal > excitation for RFP is about 585 so I 'm getting a very weak signal in the >FL2 channel. Also the YFP signal is so bright it tails into the double >positive quadrant. What is the best combination of fluorescent proteins to >use >to get optimal separation? Thanks in advance for your help. >Jackie Saleh > Aventis Pharmaceuticals > jacqueline.saleh@aventis.com > -- Mika Korkeamäki Project Engineer BioCity Turku e-mail: mika.korkeamaki@utu.fi Office: phone: +358- 2-333 7563 Dept.of Medical Microbiology mobile: +358-50-564 6855 Kiinamyllynkatu 13 fax: +358- 2-233 0008 20520 TURKU FINLAND ________________________________________________________________ Ilmainen Internet @ http://www.nic.fi/
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