We sort GFP expressing cells prettymmuch every other day. We have found
them to be relatively stable and the beauty of it all is they do not
require any antibody staining. You need only to use standard sorting
techniques for your sorts. We have noticed a bit of intensity loss in some
samples following sorting. The cells are viable and we have used them for
transplantation, cell culture and PCR following sorting.
Good luck
Charles A. Kuszynski, Ph.D.
Assistant Professor/Director
Cell Analysis Facility
University of Nebraska Medical Center
986495 Nebraska Medical Center
Omaha, NE 68198-6495
402 559-6267 voice
402 559-4077 fax
ckuszyns@unmc.edu
Hadi@pob.huji
.ac.il (Hadi) To: Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>
cc:
03/11/2001 Subject: Sorting with the GFP
06:42 AM
Dear all,
I'm planning to sort cells according to their expression of the GFP. these
cells comprise 30-40% of total poulation.
Is it possible to sort them at this percentage??
If anybody have well established protocol for this purpose i would like to
have it.
Thanks
Hadi
+--------------------------------------------------
Hadi Aslan
MSc./D.M.D. student
Laboratoy of Molecular Pathology-Prof. Dan Gazit
School of Dentistry-Hadassa Ein-karem
Hebrew University of Jerusalem
Cellular : 051-450259
Lab Tel: 02-6757625
+--------------------------------------------------
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