Hi group,
At last I can input rather than just withdraw experience....We
routinely (20.000 samples +) analyze bacteria in aqueous samples and we
have found that using a FACS scan of FACS sort it is possible to accurately
measure 100 cells/ml but that the precision falls off dramatically below
1000 cells. The precision is very much a function of the cleanliness of
the water. We have found the green SYTO (i think) stain used in molecular
probes bacterial counting kit to be very good and also using varying the
DNA stain to allow gating in FL 4 or FL 3 on the instrument if this helps
reduce the matrix background signal. I hope this helps,
james
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James A. Hall Ph.D. *** NB. Currently in Glenharvie, South
Africa *****
Research Associate, Field house 011 752 2520
Dept Geosciences cell 082 370 1229
Princeton University.
field web site
http://home.mweb.co.za/ja/jahall
Phone 609 258 2597
Fax 609 258 1274
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