I would like the flow cytometry expert's opinion on what is the industry standard for speed of centrifugation of leukemia/lymphoma samples during washing steps. There has been some debate at our institution as to whether we could use sero-fuges (~500 x g) as some flow labs do, or stick with the MMWR and vendor guidelines of spinning samples at <400 x g for 3-5 minutes. Have there been any new studies to determine if sero-fuges are acceptable? What is the danger of spinning washes too fast - aggregates? The sero-fuges would save us time, but we don't want to compromise quality if there is a known problem with spinning too fast. Thank you, Avis Peters United Hospital Flow Cytometry Lab St. Paul, MN __________________________________________________________________ Get your own FREE, personal Netscape Webmail account today at http://webmail.netscape.com/
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