sorting binucleate cells

From: Antony Bakke (bakkea@ohsu.edu)
Date: Thu Mar 01 2001 - 15:20:23 EST

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I have a PI who wants to sort binucleate from mononucleate myocytes.  The problem is that
some of the mononucleated cells clump together during preparation.  Has anyone sorted
binucleate cells from clumps of two mononucleated cells?  We only have Hoechst staining
as a marker at present, since there are no known surface markers.  The binucleates are
assumed to stop dividing and to only grow by enlarging.  The mononucleates still divide.

Thank you,
Tony Bakke, PhD
Director Oregon Cancer Center Flow Cytometry Core

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