abbas kiani wrote:
> i am working about apoptosis and Fas.Ligand and
> Bcl-2
> on mononuclear cell in blood (PBMCs)by
> flowcytometry.
> would you please lead me througth the calibration
> and optimization process.
You may find the folowing references from our laboratoyr useful for
your work in PBMC:
AIDS Res Hum Retro, 14: 1413-1422, 1998 [examines different methods for
apoptosis measurement in PBMC]
Blood, 90: 745-753, 1997 [Measures bcl-2 in an experimental PBMC based
system]
J Immunol, 158: 2456-2463, 1997 [measures fasL ina PBMC system]
Good luck,
Tom
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Thomas W. Mc Closkey, Ph. D.
Director of Flow Cytometry, North Shore University Hospital
Assistant Professor of Pediatrics, New York University School of
Medicine
Boas Marks Biomedical Research Center, 350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866
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