Dear All: I used the FDG for FACs as follows: > I injected murine stem cells marked with the bacterial beta-galactosidase > reporter gene intravenously to syngenic C3H/HeN female mice and then > 24hours post-injection i collected the cells from whole BM and spleen, > stained them with FDG and analysed them with FACs to estimate the > percentage of cells expressing b-gal, but unfourtinately i had very high > background of unspecificly FDG-stained cells (30-40% of sorted cells in > both organs) although i used the chloroquine reagent to inhibit endogenous > b-gal. > As you see, i'm trying to detect very low events, so high background is > very disturbing. > If any body had experienced similar results or have any idea to decrease > the background i would appreciate his feed back.. > Thanks, > Hadi +-------------------------------------------------- Hadi Aslan Msc./D.M.D. student Laboratoy of Molecular Pathology-Prof. Dan Gazit School of Dentistry-Hadassa Ein-karem Hebrew University of Jerusalem Cellular : 051-450259 Lab Tel: 02-6757625 +--------------------------------------------------
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