controls for leucocount procedure

From: MSchwa3722@aol.com
Date: Tue Feb 27 2001 - 13:56:30 EST


I am currently working on a flow cytometric procedure for counting residual WBC
in leuko-reduced blood bank products using Beckton-Dickinson's Leucocount Kit. The
FDA mandated control material is normal assayed blood diluted in leucoreduced whole
blood. I have had trouble with the high dilution (1:1000) required to make the control.
I'm unable to get reproducibility.  Two commercial companies have also had problems
making an FDA approvable product. Anyone out there perform this test and have any
advice on obtaining acceptable controls??



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