validation of intracellular staining with beads

From: Calman Prussin (CPRUSSIN@niaid.nih.gov)
Date: Wed Feb 21 2001 - 19:01:08 EST 

Does anyone have a reference that has compared intracellular staining
quantitation using beads and mean equivalent soluble flurochrome (MESF) with
immunologically detected intracellular protein by ELISA? These types of
validation studies have been done by Abe Schwartz and others for
EXTRACELLULAR antigens, but I could not find such a citation for
INTRACELLULAR antigens. Thanks.

Note to would be do-gooders: I am NOT looking for validation of
intracellular cytokine staining by studying secreted cytokine NOR am I
looking to validate the percent positive staining. I need to validate
intracellular protein MESF.

Surfs up, gotta' go,

Calman
> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> NIAID/ National Institutes of Health
>
>
>

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