DAPI vs PI for dead cell discrimination

From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Thu Feb 22 2001 - 23:15:42 EST 

Hi all,
	We are currently evaluating a B-D LSR in order to decide
whether it meets our requirements. One possible configuration we are
considering would use DAPI excited by the UV laser (325nm) for
live-dead discrimination in place of PI.  I know we don't need to
devote a channel to PI but some users would prefer to have a separate
live/dead channel and in most experiment we wouldn't be using the UV
laser for anything else.

My question is has anyone used DAPI for live-dead discrimination? How
does it compare to PI? Are there any particular issues we need to be
aware of?


Thanks,

Adrian Smith

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