CD19+, CD10+ surface light chain negative cells are rarely seen in significant numbers in the lymph node and even less often are a non-Hodgkin's lymphoma. Greater than 90% of follicular lymphomas are surface immunoglobulin positive. The other typical CD10 positive B cell lymphoma, Burkitt's or Burkitt like- are also surface immunoglobulin positive. The first thing I suspect when I see a large CD19+, CD10+ surface Ig- population in a lymph node- is that the light chain antibody was left out by accident. Second- that the antibody is not good. Third- staining with light chains is dim but positive. Forth- it is really negative for surface immunoglobulin. For work up when it is real-staining for bcl-2 is low on the list- after review of morphology. Morphology is clear in most cases. Maryalice >Hematogones are classically described in the bone marrow, at all >ages. There may >be circulating hematogones in the peripheral blood of neonates. >Circulating CD10+ >CD19+ CD34+ cells circulating in the blood in a patient past the >neonatal period >might represent leukemic blasts. CD10+, CD19+, surface light chain >negative cells in >a lymph node represent follicle center cells and might be lymphoma; >stains could be >done for bcl-2. > >Tim Singleton, MD >William Beaumont Hospital >Royal Oak, MI > > >>> sterling stoudenmire <sstouden@thelinks.com> 02/07/01 08:55PM >>> > >andrea, some of these markers might be specific to the location from which >the sample was taken? any comments on that issue? sterling > >At 01:12 PM 2/5/01 -0500, Andrea Illingworth wrote: > > Another question for the flow experts: of B-cell differentiation: > >CD45 dim, very low FALS Pop#2: CD19+, CD10+ (a little weaker than the > >first pop.), CD34-, weak CD20 and CD22, no k/l, CD45 ( a little brighter > >than pop 1), very low FALS Pop#3: CD19+, CD20+ (bright), CD22+ (bright), > >polyclonal kappa and lambda, bright CD45+ Which ones are the > >hematogones, only the first population or is the second population > >(CD10+,CD34-) also called hematogones? What is the definition of > >hematogones? Are they always CD34+? 1 and 2, they seem to "jump" from > >being CD34+ to being CD34 negative without the stages in between. Thank > >you for any insight Andrea Illingworth DCDS Flow Cytometry Bangor, Maine >Computer Aided Cell and Molecular Biology (CACMB), not medicine, will find >the cure for cancer and other diseases. There will always be a need for >the trained clinician (MD/RN) but, advanced diagnostic and treatment option >selection has become gene based, has moved from the physician's practice to >the computerized cell and molecular biology laboratory, and appropriate >treatment options should now be based on the personal biology of the >patient. Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH "Learn the rules so you know how to break them properly." The Dalai Lama
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