Many moons ago, we investigated "sea squirt" blood cells with flow cytometry. While not quite bivalves, they're closely related. We were able to resolve multiple cell types on the basis of autofluorescence and light scatter. It was a very interesting project. I would caution you on comparing light scatter patterns from chordate blood to mammalian cells. The functional roles of the chordate cells are undoubtedly quite different from mammalian. One of the goals in the citation below was to determine the cell population(s) responsible for sequestering large amounts of vanadium. good luck, td Oltz EM; Pollack S; Delohery T; Smith MJ; Ojika M; Lee S; Kustin K; Nakanishi K Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. Department of Chemistry, Columbia University, New York, New York 10027. Experientia 1989 Feb 15;45(2):186-90 >Hi, > >How blood cells are formed in bivalves is unknown. In fact, most >things about bivalve blood cells remain unknown. Using flow >cytometry, we have sorted a few subpopulations (that we can see with >light scatter) and described them. What we would like to know is; if >we see 3 subpopulations, 1 resembling granulocytes another resembling >lymphocytes, with a 3rd that has an intermediate appearance >(non-granulated granulocytes and/or non-granulocytes with vacuoles) >and placement on a histogram, is there any reason to believe that >this intermediate population could be a precursor population to >granulocytes? Please excuse my ignorance of proper terminology. >Thank you. > >Kathy >-- >Kathryn A. Ashton-Alcox >Haskin Shellfish Research lab >Rutgers University >6959 Miller Avenue >Port Norris, NJ 08349 >USA > >856-785-0074 x122 >856-785-1544 fax > >kathryn@hsrl.rutgers.edu -- -- ============================================================================== Thomas M. Delohery |Internet: t-delohery@ski.mskcc.org Supervisor, Flow Cytometry Core Facility | Office Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Lab Phone: (212) 639-5162 1275 York Ave. Box 98 | Fax: (917) 432-2333 New York, NY 10021 | ==============================================================================
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