Re: Labeling Ab with Alexa 594

From: Nicole Baumgarth (nbaumgarth@UCDAVIS.EDU)
Date: Mon Feb 05 2001 - 17:27:33 EST

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In reply to: David Dombkowski: "Labeling Ab with Alexa 594"
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David,
I have not labeled SCA-1 with Alexa 594, but many other antibodies (mostly
rat anti-mouse). They worked really well when excited with a dye laser
tuned to give a 595nm laser line (150mW). Just follow the instructions -
worked every time for us
Nicole

> Does anyone have experience labeling an Ab with Alexa 594. We want to
>label Sca-1 with Alexa 594 using the Molecular Probes kit. Any tips. Thanks.
>
>David M. Dombkowski
>dombkowski@helix.mgh.harvard.edu
>Flow Cytometry-Pathology-CNY rm7017
>149 13th Street
>Massachusetts General Hospital-East
>Boston, MA 02129
>Tel. (617)726-1683
>Fax.(617)724-3164


Nicole Baumgarth
Center for Comparative Medicine
University of California
Davis, CA 95616
Tel:   530 - 754 5813 (office)
         530 - 754 6538 (lab)
FAX: 530 - 752 7914

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