Dear all, I am using the nucleic acid stain SYTO13 on natural aquatic samples to investigate the bacteria and phytoplankton populations. However, whilst comparing stained and unstained samples, it seems that some of the phytoplankton populations have somewhat shrunk (i.e they have lower FLS values). The extent of the reduction in FLS,however,is different for different populations. I was wondering if anyone has encountered similar problems with the SYTO family of nucliec acid stains? Regards Neo Sim yee, Lezlie National University of Singapore
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