Urgent, please. Unclumped PBMCs

From: Gaffin, Stephen L USARIEM (Stephen.Gaffin@na.amedd.army.mil)
Date: Tue Jan 30 2001 - 15:40:46 EST


Dear flowers.
	Can anyone supply a method / resuspension buffer to produce
monodisperse ("unclumped") PBMCs after they have been spun down at 1,000
rpms 3 or 4  times?
	Thank you thank you in advance for this urgently needed method.
Steve


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** Stephen L. Gaffin, Ph.D.				   **
** Research Physiologist				   **
** U.S. Army Research Institute for Environmental Medicine **
** Natick MA  01760-5007				   **
** Tel. 508-233-4867	   Fax 508-233-4869		   **
** email  stephen.gaffin@na.amedd.army.mil		   **
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