How low can LIGAND DENSITY go?

From: Calman Prussin (CPRUSSIN@niaid.nih.gov)
Date: Wed Jan 31 2001 - 15:57:43 EST 

While we are on the subject of counting angels on the heads of pins:

In addition to limiting frequencies of positive cells, LIGAND DENSITY can
also be a problem. My 2 part question is:

1. What is the lower limit of detection of surface molecules using a bench
top flow cytometer and typical staining, compensation techniques? Units can
be in molecules of FITC or PE, # of receptors, angels, etc.

2. What techniques have you personally used to enhance this lower limit of
detection and what S/N enhancement did you get?

Thanks,

Calman Prussin
> Laboratory of Allergic Diseases
> NIAID/ National Institutes of Health
>
>

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