Just wanted to say a big thanks to the multitudes that have responded to my question about anti-CD3. The big "winner" (in terms of number of recommendations) by far was the FN-18 clone from Biosource. I had actually tried that antibody in the past with decent staining for rhesus but lousy staining for cynos - but that was with a "home-made" CD3-Cy5 conjugate. The FITC version that was recommended may give better results. I did hear from a number of people that Pharmingen is having trouble with the PerCP conjugation of SP34, not the SP34 itself - so we will probably try ordering some of that in a different "flavor" and fit it around our needs, based on my previous luck with FN-18. It sounds as though Diatec also has a potential clone which may be of use. Again, many thanks to all who replied! Sincerely, Doug Douglas S. Reed, Ph.D. Department of Aerobiology and Product Evaluation Division of Toxinology & Aerobiology U.S. Army Medical Research Institute of Infectious Diseases 1425 Porter St. Ft. Detrick Frederick, MD 21702 (301) 619-6728 voice (301) 619-2541 fax doug.reed@det.amedd.army.mil
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