I have a lot of fcs files from old experiments where FL3 CD45 was not used for purity. These files include erros in lysis, nucleated RBC's, thalassemiacs, thrombocytopeniacs and neonates (all notorious for low purity with 2 color FACS). I created a template which does not need the CD45 information and extracts phenotypes for CD's 3,4,8,16+56 and 19 directly from the fluorescence of those markers. In my tests the results are very close to the normal analysies. But because they're based on assumptions, there is room for error. I created these for use in a lab that does not have 3 color capacity or is doing retrospect analysis like me. The assumptions are: NK+B+T=100% lymphocytes and CD4+CD8= 100% CD3 anyway, I posted the template along with a bit of an overview on my site. play around with it if it's useful and send me feedback. I begun to post the template and description on 1/25/01, should be ready by the next day. http://www.cd4cd8.com/method/ maciej PS this is work in progress :) email me if you have suggestions. __________________________________________________ Do You Yahoo!? Yahoo! Auctions - Buy the things you want at great prices. http://auctions.yahoo.com/
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